Определение группы крови по системе ABO при помощи стандантных сывороток - ABO-typing test

Before starting work with body fluids (like blood) it is necessary to wear protective clothing: mask, cap, glasses, gloves, apron. ABO-typing test. Equipment: white plate with cells for serums; standard serums of two series, a sample of analyzed blood, glass slides or sticks for mixing the blood and serums, saline (0,9% NaCl), hourglass on 5 minutes or timer. Algorithm of the test: ABO-typing should be carried out in the room with temperatures ranging from 15 to 25̊ С. 1. Plate should be marked with the patient`s name. 2. Checking the expiry dates and appearance of serums. 3. Put one big drop of О, А and В serums of two different series to six cells. 4. Add to each serum one little drop (ratio 1:10) of analyzed blood by sticks or corners of the glass slide and mix. 5. Wiggle the plate. 6. After 3 minutes put inside each cell with visible agglutination 1 drop of saline (to prevent false agglutination). 7. Wiggle the plate up to 5 minutes. 8. After 5 minutes check presence of agglutination (flakes from RBC): absence of agglutination in all cells means the first group О(I); agglutination with serums O and В – the second group А(II); agglutination with serums O and А – the third group В(III). In agglutination inside all 6 cells we should perform the test with АВ-serum; absence of agglutination means that tested group is the fourth АВ(IV). Перед тем, как приступить к работе с биологическими жидкостями, необходимо надеть защитную одежду: маску, шапочку, очки, перчатки, фартук....