Pci express x16 Version 3.0 vs 4.0 vs 2.0 | RTX 3080

Is there any unbelievable Differences Between PCI Express 3.0, 4.0 & 2.0 with the RTX 3080 ? Test Details Latest & old games tested Geforce 527.37 driver used. ambient temp 28 - 29 deg , 1920 x 1080resolution. CPU - AMD R5 5600x stock speed CPU Cooler - gigabyte 280 waterforce GPU - gigabyte turbo rtx 3080 10gb ddr6x Motherboard - MSI x570 gaming edge wifi RAM 2x 8GB - Crucial ballistix sport 3600 CL16 (16-18-18-38) , 2 x 8gb CASE - Antec NX 800 , galax rev 02 Power - antec neo 650 watts power supply NVMe/SSD - (OS) WD SN810 , gigabyte ssd - 480GB / 1TB HDD - 2TB Western Digital 7200 rpm OS - Windows 10 pro build 21H2 Monitor - Acer cb281 , LG 34ul500 vmreviews vmreview80@gmail.