Western Blot Part 1

This tutorial video introduces one of my favorite lab techniques, western blotting. Western blot technique is used to isolate, visualize, and study proteins. This is the 1st part of a series of western blot technique videos I will be uploading. Time stamps 0:00 Introduction to western blot technique 5:27 Materials for lysing cells 7:10 Washing cells with 1XTBS 8:40 Aspirating TBS (washing buffer) completely 10:04 Adding lysing buffer (RIPA) 10:51 Front view of lysing cells with scraper 11:40 Top view of lysing cells with scraper 13:00 Relaxing side view of lysing cells with lofi music 18:35 Centrifuging cell lysates 19:08 Collecting cell supernatant without the cell pellet 21:43 Preparing protein samples for BCA, DF=1/3 25:36 2nd example of preparing protein samples for BCA, DF=1/4 28:11 BCA materials and protocol 30:35 Pipetting protein standards and samples into 96-well plate for BCA 39:10 Calculations to determine amounts of reagents A and B 42:20 Top view or pipetting 200uL of reagents A and B mixture 43:28 Close up view of pipetting 200uL of reagents A and B mixture 44:03 Shaking 96-well plate for incubation at 37C